400m Women Semi Finals, Heat:2 of 3 ; Official result since 13:31
| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
| 1 | 8 | 353 | Omotosho Omolara | NSCDC | 53.05 | Q | |||
| 2 | 5 | 375 | Opeyemi Oke Deborah | Ondo | 53.30 | Q | |||
| 3 | 3 | 136 | Esther J. JOSEPH | Delta | 53.49 | q | |||
| 4 | 6 | 378 | Ada Bright | Osheku | 54.29 | ||||
| 1 | 22 | Success DURUZOR | FB | DNS | |||||
| 2 | 100 | Olusesan Temitope | Customs | DNS | |||||
| 5 | 15 | Knowledge Omovoh | FB | DNS | |||||
| 7 | 142 | Joseph Esther | Delta | DNS |