400m Women Heats, Heat:1 of 3 ; Official result since 17:31
| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
| 1 | 7 | 748 | Joseph Esther | Delta | 53.39 | ||||
| 2 | 3 | 323 | Olotu Folashade | Ekiti | 55.25 | ||||
| 3 | 1 | 87 | Akintoye Blessing  Jo | D'victors | 55.74 | ||||
| 4 | 2 | 614 | Omolara Omotoso-Ogunmakinju | NSCDC | 56.25 | ||||
| 5 | 5 | 393 | Verity Umaru | Police | 57.10 | ||||
| 6 | 6 | 682 | Mpama Star | Unattached | 57.73 | ||||
| 7 | 4 | 68 | Alowonle Alimot | Olapade | 58.18 | ||||
| 8 | 8 | 685 | Nwachukwu Bosede Veronica | FUTA | 61.43 |